GAO Juanjuan, ZHAO Mengling, SHAN Shujun, LI Yongyi, LUO Jun, LI Yi. Rapid identification of stigmastane-type steroid saponins from Vernonia amygdalina leaf based on α-glucosidase inhibiting activity and molecular networking [J].Chin J Nat Med, 2022, 20(11): 846-853. doi: 10.1016/S1875-5364(22)60235-8
Citation: GAO Juanjuan, ZHAO Mengling, SHAN Shujun, LI Yongyi, LUO Jun, LI Yi. Rapid identification of stigmastane-type steroid saponins from Vernonia amygdalina leaf based on α-glucosidase inhibiting activity and molecular networking [J].Chin J Nat Med, 2022, 20(11): 846-853. doi: 10.1016/S1875-5364(22)60235-8

Rapid identification of stigmastane-type steroid saponins from Vernonia amygdalina leaf based on α-glucosidase inhibiting activity and molecular networking

  • Steroid saponins are secondary metabolites with multiple medicinal values that are found in large quantities in natural medicines, especially Vernonia amygdalina, a famous nature medicine for the treatment of tonsillitis, diabetes, pneumonia. The current study was designed to combine molecular networking (MN) with diagnostic ions for rapid identification of Δ7,9(11) stigmastane-type saponins which were the α-glucosidase inhibitory active substances in V. amygdalina. First, the α-glucosidase inhibitory activities of five Δ7,9(11) stigmastane-type steroid saponins that were previously isolated were screened, which indicated that the Δ7,9(11) stigmastane-type steroid saponin was one of the active constituents responsible for ameliorating diabetes. Furthermore, a strategy was proposed to identify stigmastane-type steroid saponins and verify the plausibility of derived fragmentation pathways by applying MN, MolNetEnhancer and unsupervised substructure annotation (MS2LDA). Based on this strategy, other seven Δ7,9(11) stigmastane-type steroid saponins were identified from this plant. Our research provide scientific evidence for the antidiabetic potential of the steroid saponin-rich extract of V. amygdalina leaf.
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