Effects of salvianolic acid and notoginseng triterpenes on angiogenesis in EA-hy926 cells in vitro

AIM:To investigate the different effects of salvianolic acid and notoginseng triterpenes on proliferation,angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in vitro.METHODS:EA-hy926 cells were cultured in vitro.Salvianolic acid and notoginseng triterpenes at concentrations of 0.4,0.8 and 1.2 mg·L-1 were used to culture EA-hy926 cells.EA-hy926 cells in a blank control group were grown in culture solution only.Viability of cells was assessed by CCK-8,and after treated for 12 h,capillary-like structures were examined.After 24 h culture,the expression of VEGF was detected by real-time PCR.RESULTS:Salvianolic acid at 0.4,0.8 mg·L-1,the same as notoginseng triterpenes,increased VEGF content in EA-hy926 cells.Expression of VEGF protein in the salvianolic acid at 1.2 mg·L-1 group,was up-regulated as compared with notoginseng triterpenes group(PCONCLUSION:Salvianolic acid and notoginseng triterpenes can promote EA-hy926 cell proliferation,angiogenesis and expression of VEGF protein.This analysis also provided evidence that salvianolic acid had the better effects as compared with notoginseng triterpenes.