Abstract:
Withaminimas A-F (
1-6 ), six new withaphysalin-type withanolides were isolated from the aerial parts of
Physalis minima L.. The structures of these compounds were elucidated through a variety of spectroscopic techniques including HR-MS, NMR, and ECD. Compound
1 belongs to rare 18-norwithanolides, and
2-3 were 13/14-secowithanolides. According to the traditional usage of
P. minima, inhibitory effects on nitric oxide (NO) production in lipopolysaccaride-activated RAW264.7 macrophages were evaluated, and compounds
1-4 exhibited significant inhibitory effects with IC
50 values among 3.91-18.46 μmol·L
-1.