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JIANG Lei, XUE Yan-Jiu, LIU Wei-Ying, MA Min, WU Xu-Ri, WANG Shu-Zhen, CHEN Yi-Jun. The importance of start codon of nosM in nosiheptide production[J]. 中国天然药物, 2015, 13(11): 854-860.
引用本文: JIANG Lei, XUE Yan-Jiu, LIU Wei-Ying, MA Min, WU Xu-Ri, WANG Shu-Zhen, CHEN Yi-Jun. The importance of start codon of nosM in nosiheptide production[J]. 中国天然药物, 2015, 13(11): 854-860.
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JIANG Lei, XUE Yan-Jiu, LIU Wei-Ying, MA Min, WU Xu-Ri, WANG Shu-Zhen, CHEN Yi-Jun. The importance of start codon of nosM in nosiheptide production[J]. Chinese Journal of Natural Medicines, 2015, 13(11): 854-860.
Citation: JIANG Lei, XUE Yan-Jiu, LIU Wei-Ying, MA Min, WU Xu-Ri, WANG Shu-Zhen, CHEN Yi-Jun. The importance of start codon of nosM in nosiheptide production[J]. Chinese Journal of Natural Medicines, 2015, 13(11): 854-860.
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The importance of start codon of nosM in nosiheptide production

The importance of start codon of nosM in nosiheptide production

    摘要
  • 摘要: The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.

     

    Abstract: The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.

     

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