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CHEN Jin-Jin, ZHAO Qing-Sheng, LIU Yi-Lan, ZHA Sheng-Hua, ZHAO Bing. Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence[J]. 中国天然药物, 2015, 13(9): 653-659.
引用本文: CHEN Jin-Jin, ZHAO Qing-Sheng, LIU Yi-Lan, ZHA Sheng-Hua, ZHAO Bing. Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence[J]. 中国天然药物, 2015, 13(9): 653-659.
CHEN Jin-Jin, ZHAO Qing-Sheng, LIU Yi-Lan, ZHA Sheng-Hua, ZHAO Bing. Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence[J]. Chinese Journal of Natural Medicines, 2015, 13(9): 653-659.
Citation: CHEN Jin-Jin, ZHAO Qing-Sheng, LIU Yi-Lan, ZHA Sheng-Hua, ZHAO Bing. Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence[J]. Chinese Journal of Natural Medicines, 2015, 13(9): 653-659.

Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence

Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence

  • 摘要: Maca (Lepidium meyenii) is an herbaceous plant that grows in high plateaus and has been used as both food and folk medicine for centuries because of its benefits to human health. In the present study, ITS (internal transcribed spacer) sequences of forty-three maca samples, collected from different regions or vendors, were amplified and analyzed. The ITS sequences of nineteen potential adulterants of maca were also collected and analyzed. The results indicated that the ITS sequence of maca was consistent in all samples and unique when compared with its adulterants. Therefore, this DNA-barcoding approach based on the ITS sequence can be used for the molecular identification of maca and its adulterants.

     

    Abstract: Maca (Lepidium meyenii) is an herbaceous plant that grows in high plateaus and has been used as both food and folk medicine for centuries because of its benefits to human health. In the present study, ITS (internal transcribed spacer) sequences of forty-three maca samples, collected from different regions or vendors, were amplified and analyzed. The ITS sequences of nineteen potential adulterants of maca were also collected and analyzed. The results indicated that the ITS sequence of maca was consistent in all samples and unique when compared with its adulterants. Therefore, this DNA-barcoding approach based on the ITS sequence can be used for the molecular identification of maca and its adulterants.

     

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