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王小彦, 马增春, 邵帅, 洪倩, 王宇光, 谭洪玲, 路晓钦, 董志, 高月. 腺嘌呤对电离辐射诱发AHH-1细胞凋亡的保护作用[J]. 中国天然药物, 2013, 11(2): 139-144.
引用本文: 王小彦, 马增春, 邵帅, 洪倩, 王宇光, 谭洪玲, 路晓钦, 董志, 高月. 腺嘌呤对电离辐射诱发AHH-1细胞凋亡的保护作用[J]. 中国天然药物, 2013, 11(2): 139-144.
shu
WANG Xiao-Yan, MA Zeng-Chun, SHAO Shuai, HONG Qian, WANG Yu-Guang, TAN Hong-Ling, LU Xiao-Qin, DONG Zhi, GAO Yue. Radioprotective effect of adenine on irradiation-induced apoptosis[J]. Chinese Journal of Natural Medicines, 2013, 11(2): 139-144.
Citation: WANG Xiao-Yan, MA Zeng-Chun, SHAO Shuai, HONG Qian, WANG Yu-Guang, TAN Hong-Ling, LU Xiao-Qin, DONG Zhi, GAO Yue. Radioprotective effect of adenine on irradiation-induced apoptosis[J]. Chinese Journal of Natural Medicines, 2013, 11(2): 139-144.
shu

腺嘌呤对电离辐射诱发AHH-1细胞凋亡的保护作用

Radioprotective effect of adenine on irradiation-induced apoptosis

    摘要
  • 摘要: 目的:观察腺嘌呤对淋巴母细胞AHH-1辐射损伤的保护作用并探讨其作用机制。方法:照射前12 h给予不同浓度的腺嘌呤,再经4.0 Gy 60Co射线照射诱发细胞凋亡,采用MTS法计算细胞存活率;Annexin V-FITC/PI双染法、Caspase3/7发光法检测细胞凋亡率;DNA Ladder法观察凋亡断裂条带;RT-PCR分析细胞Caspase-9、Bax、Bcl-2 mRNA表达的变化。结果:与正常组比较,电离辐射后细胞存活率79.4%显著下降(P0.01,加入0.001,0.01,0.1 molL-1腺嘌呤细胞存活率分别为85.4%,89.6%,92.2%明显升高(P0.01),且随作用浓度的增加而增大,DNA断裂片段也减少,细胞中Caspase-9、Bax表达明显减少,Bcl-2表达明显增加,凋亡的各项指标均明显改善。结论:腺嘌呤对电离辐射诱发的细胞凋亡有保护作用,并且对细胞凋亡的抑制呈剂量依赖正相关关系。

     

    Abstract: AIM:To investigate the radioprotective effect of adenine on irradiated lymphocytes and discover the possible mechanisms of protection.METHODS:Lymphocytes were pretreated for 12 h with adenine(0.001-0.1 molL-1) and then exposed to 4 Gy radiation.Cell viability was observed by the MTS assay,apoptosis was detected by Annexin V-FITC/PI,DNA ladder,and caspase 3/7 activity.Caspase-9,Bax,and Bcl-2 gene expression was investigated by RT-PCR.RESULTS:Irradiation increased cell death and DNA fragmentation.Pretreatment with adenine significantly reversed this tendency.Furthermore,several anti-apoptotic characteristics of adenine were determined,including the ability to inhibit caspase 3/7,upregulate B-cell lymphoma(Bcl-2) and downregulate Bcl-2-associated X(Bax),capase-9 gene expression in 4 Gy-irradiated AHH-1 cells.CONCLUSION:The results suggest that adenine had a radioprotective effect to inhibit apoptosis in a concentration dependent manner.

     

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