• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库（CSCD）
  • 中国科技论文与引文数据库（CSTPCD）
  • 中国学术期刊文摘数据库（CSAD）
  • 中国学术期刊（网络版）（CNKI）
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库（SCOPUS）
  • 日本科学技术振兴机构数据库（JST）
高级检索
杨光明, 燕茹, 王兆先, 张芳芳, 潘扬, 蔡宝昌. 镰形棘豆提取物抗肝癌的体内外活性研究[J]. 中国天然药物, 2013, 11(5): 519-524.
引用本文: 杨光明, 燕茹, 王兆先, 张芳芳, 潘扬, 蔡宝昌. 镰形棘豆提取物抗肝癌的体内外活性研究[J]. 中国天然药物, 2013, 11(5): 519-524.
shu
YANG Guang-Ming, YAN Ru, WANG Zhao-Xian, ZHANG Fang-Fang, PAN Yang, CAI Bao-Chang. Antitumor effects of two extracts from Oxytropis falcata on hepatocellular carcinoma in vitro and in vivo[J]. Chinese Journal of Natural Medicines, 2013, 11(5): 519-524.
Citation: YANG Guang-Ming, YAN Ru, WANG Zhao-Xian, ZHANG Fang-Fang, PAN Yang, CAI Bao-Chang. Antitumor effects of two extracts from Oxytropis falcata on hepatocellular carcinoma in vitro and in vivo[J]. Chinese Journal of Natural Medicines, 2013, 11(5): 519-524.
shu

镰形棘豆提取物抗肝癌的体内外活性研究

Antitumor effects of two extracts from Oxytropis falcata on hepatocellular carcinoma in vitro and in vivo

    摘要
  • 摘要: 目的:探讨镰形棘豆提取物的体内外抗肝癌活性,并探讨其可能的机制。方法:采用MTT法检测镰形棘豆提取物对SMMC-7721细胞的增殖抑制,采用PI单染法和Annexin V-FITC/PI双染法进行流式细胞仪检测,测定细胞周期和凋亡率,观察H22荷瘤小鼠的体内肿瘤抑制作用。结果:MTT实验表明,镰形棘豆提取物EOOF和FOF有效抑制肿瘤细胞SMMC-7721的增殖,并呈一定的剂量依赖性,其IC50值分别为0.115和0.097 mg·mL-1。TFOF作用SMMC-7721细胞后可使细胞周期停滞于G1期,凋亡细胞的比例升高,并且呈浓度依赖性。镰形棘豆提取物FOF能明显抑制H22荷瘤小鼠肿瘤的生长,低、高剂量组的抑制率分别为56.1%和70.8%(P结论:镰形棘豆提取物FOF能抑制SMMC-7721细胞的增殖,诱导其凋亡,并对H22荷瘤小鼠有肿瘤生长抑制作用。显示镰形棘豆具有较好的发展为抗肝癌药物的前景。

     

    Abstract: AIMS:To investigate the antitumor effects of extracts from Oxytropis falcata on human hepatocellular carcinoma SMMC-7721 cells in vitro and in transplanted murine H22 tumors in vivo.METHODS:Cell proliferation,cell cycle distribution and apoptosis in SMMC-7721 cells were determined and tumor growth inhibition in H22 tumors was investigated.Cell cycle distribution was analyzed by flow cytometry with propidium iodide(PI) and Annexin V-FITC/PI double staining.RESULTS:MTT assay revealed that essential oil and flavonoids of O.falcata(named EOOF and FOF) inhibited proliferation of SMMC-7721 cells in a dose-dependent manner.The IC50 value of EOOF and FOF were 0.115 and 0.097 mg·mL-1,respectively.Cell cycle was arrested at G1 phase,and induction of apoptosis occurred in SMMC-7721 cells when subjected to FOF.Growth inhibition in H22 solid tumors transplanted mice was significantly pronounced after being treated with FOF,and the inhibition ratio were 56.1% and 70.8% at the concentration of 30 and 60 mg·kg-1.CONCLUSION:The results suggest that FOF promotes apoptosis in SMMC-7721 cells and inhibits H22 tumor growth,resulting in a potential antitumor effect on hepatic cancer.

     

    • HTML全文
    • 参考文献(25)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回