千里香枝中39个多甲氧基黄酮的HPLC-DAD-ESI-MS/MS分析

Characterization of thirty-nine polymethoxylated flavonoids(PMFs) in the branches of Murraya paniculata by HPLC-DAD-ESI-MS/MS

摘要: 目的:定性分析千里香枝中的多甲氧基黄酮类成分。方法:建立一种灵敏的HPLC-DAD-ESI-MS/MS方法,筛选千里香枝中的多甲氧基黄酮。结果:分别研究总结了多甲氧黄酮、二氢黄酮、查耳酮以及多甲氧基黄酮苷的诊断性断裂途径。结合这些特征和EIC-MS/MS实验,筛选了39个多甲氧基黄酮类成分,包括24个多甲氧基黄酮、10个多甲氧基二氢黄酮或查耳酮、5个多甲氧基黄酮苷。结论:本研究为从复杂物质体系中筛选多甲氧基黄酮提供了一种快速有效的方法。

Abstract: AIM:To investigate the polymethoxylated flavonoids(PMFs) in the branches of Murraya paniculata(L.) Jack.METHODS:A sensitive HPLC-DAD-ESI-MS/MS method was established to screen PMFs in the branches of M.paniculata based on the analysis of six PMF standards in the positive mode by CID-MS/MS.RESULTS:The diagnostic fragmentation pathways for polymethoxylated flavones,polymethoxylated flavanones,polymethoxylated chalcones and PMF glycosides were summarized,respectively.According to the MS fragmentation pathways,39 PMFs,including 24 flavones,10 flavanones or chalcones and 5 PMFs glycosides were screened.CONCLUSION:The results indicated that the developed analytical method could be employed as a rapid,effective technique for the chemical screening of PMFs in TCMs extracts.