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李文兰, 白晶, 胡杨, 季宇彬, 杜娟, 孙志. HPLC-ESI/MSn鉴定金匮肾气丸总苷在大鼠尿及粪中的代谢产物[J]. 中国天然药物, 2013, 11(1): 56-62.
引用本文: 李文兰, 白晶, 胡杨, 季宇彬, 杜娟, 孙志. HPLC-ESI/MSn鉴定金匮肾气丸总苷在大鼠尿及粪中的代谢产物[J]. 中国天然药物, 2013, 11(1): 56-62.
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LI Wen-Lan, BAI Jing, HU Yang, JI Yu-Bin, DU Juan, SUN Zhi. Screening and analysis of metabolic components in rat urine and feces after oral administration of total glycosides of Jinkuishenqi pills by HPLC-ESI/MSn[J]. Chinese Journal of Natural Medicines, 2013, 11(1): 56-62.
Citation: LI Wen-Lan, BAI Jing, HU Yang, JI Yu-Bin, DU Juan, SUN Zhi. Screening and analysis of metabolic components in rat urine and feces after oral administration of total glycosides of Jinkuishenqi pills by HPLC-ESI/MSn[J]. Chinese Journal of Natural Medicines, 2013, 11(1): 56-62.
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HPLC-ESI/MSn鉴定金匮肾气丸总苷在大鼠尿及粪中的代谢产物

Screening and analysis of metabolic components in rat urine and feces after oral administration of total glycosides of Jinkuishenqi pills by HPLC-ESI/MSn

    摘要
  • 摘要: 应用高效液相色谱-电喷雾离子阱质谱法(HPLC-ESI/MSn)鉴定金匮肾气丸总苷的化学成分及总苷大鼠灌胃给药尿粪化学成分或代谢产物。对比体内外样品色谱图各色谱峰,根据负离子模式下的分子离子峰获得化合物分子量信息。金匮肾气丸总苷含19个化学成分,分别为没食子酰-3-O-芹糖基(16)葡糖苷、羟基芍药苷、莫诺苷、马钱苷、獐芽菜苷、地黄苷A或B、1,2,3-三-O-没食子酰葡萄糖、芍药苷、山茱萸新苷Ⅱ、6'-(3,4,5-三羟基苯甲酰)芍药苷、1,2,3,6-四-O-没食子酰葡萄糖、山茱萸新苷Ⅰ、五没食子酰葡萄糖、苯甲酰羟基芍药苷、丹皮酚原苷、苯甲酰芍药苷、4'-羟基,6'-(3,4,5-三羟基苯甲酰)芍药苷和两个未知化合物成分。金匮肾气丸总苷大鼠灌胃尿液鉴定出18个化学成分,其中有9个化合物以原型存在,9个可知代谢转化成分,分别为分别为没食子酸,脱水莫诺苷元,2-羟基苯乙酮-4-O-葡萄糖醛酸酯,芍药苷代谢素,1-O-甲基,3-羟基马钱苷元,马钱苷元,2-羟基-4-甲氧基苯乙酮-5-O-硫酸酯,2,4-二羟基苯乙酮和1个未知成分。金匮肾气丸总苷大鼠灌胃粪便鉴定出20个化学成分,其中有12个化合物以原型存在,8个可知代谢转化成分,分别为没食子酸,獐芽菜苷代谢素A,芍药苷代谢素Ⅰ,1-O-甲基,3-羟基马钱苷元,还原型莫诺苷元,马钱苷元和2个未知成分。HPLC-ESI/MS能够高效的分离、灵敏的检测和全面的鉴定总苷的原型及代谢转化成分,对揭示化学成分在体内的代谢机制具有重要意义。

     

    Abstract: AIM:To identify the chemical components of the total glycosides(TG) of Jinkuishenqi pills and their metabolites in vivo.METHODS:An HPLC-ESI-MS method was developed for analysis of the metabolites of TG in rat plasma,after the rat was administered with the TG.RESULTS:A total of 18 compounds were detected in urine after oral administration of TG,nine of which are metabolites and the others are the original form of the compounds contained in Jinkuishenqi pills.Among them,the metabolites are gallic acid,dehydromorroniaglycone,2-hydroxyacetophenone-4-O-glucuronic acid esters,paeonimetabolin,Mlog Ⅰ-2,Mlog-1,2-hydroxy-4-methoxy acetophenone-5-O-sulfate,2,4-dihydroxyacetophenone and one unknown compound,respectively.A total of 20 compounds were detected in the feces after oral administration of TG,eight of which are metabolites and the others are the original form of the compounds contained in Jinkuishenqi pill.Among them,the metabolites are gallic acid,swerosimetabolin A,paeonimetabolin,Mlog Ⅰ-2,Mmor-2,Mlog-1 and two unknown compounds.CONCLUSIONS:Using HPLC-ESI-MS,it was possible to screen and analyze the multiple compounds and metabolites of TG in vivo of rats.The identification and structure elucidation of these metabolites provided essential data for further pharmacological and mechanism of effectiveness of TG.

     

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