冠心宁注射液中主成分在线HPLC-DAD-CL抗氧化活性测定
On-line antioxidant activity determination of main ingredients in Guan-Xin-Ning injection by HPLC-DAD-CL
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摘要: 采用HPLC-DAD-CL方法同时分析了冠心宁注射液中15个成分的抗氧化活性。冠心宁注射液是由丹参和川芎组方的传统中药制剂,主要用于治疗冠心病和心绞痛。该分析方法的条件被优化、验证,并用于14批冠心宁注射液中15个酚酸成分的H2O2抑制率测定,结果显示15个化合物的抗氧化活性具有明显的差异,且丹参素、原儿茶醛和迷迭香酸是冠心宁注射液整体抗氧化活性的主要贡献成分(25.82%-51.19%)。此外,通过PCA方法可将14批冠心宁注射液分成5组,此5组与5个生产厂家对应。因此,本论文提出的HPLC-DAD-CL方法简单、快速、成本低,可用于复杂体系的抗氧化成分的筛选、揭示冠心宁注射液的抗氧化机制,亦可从活性角度用于其质量控制。Abstract: A novel on-line method using high performance liquid chromatography (HPLC)with a diode array detector (DAD) and a chemiluminescence (CL) detector was successfully developed for simultaneously analyzing the antioxidant efficacies of 15 components in Guan-Xin-Ning (GXN) injection, a traditional Chinese medicinal preparation consisting of Radix Salvia miltiorrhiza and Rhizoma Ligusticum chuanxiong for the treatment of coronary heart disease and angina pectoris. The analytical conditions were optimized, validated and applied to determine H2O2 inhibition rates of the 15 phenolic acids in GXN injections from 14 different production batches. The results showed that antioxidant diversities were found in the 15 compounds and that danshensu, protocatechuic aldehyde and rosmarinic acid were largely responsible for the antioxidant activity of GXN injection (25.82%-51.19%). In addition, 14 batches of GXN injections were divided into five groups corresponding with five different factories by principal component analysis (PCA) assay. Therefore, the proposed HPLC-DAD-CL method was simple, rapid and low-cost for screening antioxidant constituents in a complex system, which can be used to reveal the antioxidant mechanism of GXN injection and is applicable for its quality control in terms of activity, which was different from the routine chemical assessment.