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Sardar Atiq Fawad, Nauman Khalid, Waqas Asghar, Hafiz Ansar Rasul Suleria. in vitro comparative study of Bougainvillea spectabilis "stand" leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity[J]. 中国天然药物, 2012, 10(6): 441-447.
引用本文: Sardar Atiq Fawad, Nauman Khalid, Waqas Asghar, Hafiz Ansar Rasul Suleria. in vitro comparative study of Bougainvillea spectabilis "stand" leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity[J]. 中国天然药物, 2012, 10(6): 441-447.
Sardar Atiq Fawad, Nauman Khalid, Waqas Asghar, Hafiz Ansar Rasul Suleria. in vitro comparative study of Bougainvillea spectabilis "stand" leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity[J]. Chinese Journal of Natural Medicines, 2012, 10(6): 441-447.
Citation: Sardar Atiq Fawad, Nauman Khalid, Waqas Asghar, Hafiz Ansar Rasul Suleria. in vitro comparative study of Bougainvillea spectabilis "stand" leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity[J]. Chinese Journal of Natural Medicines, 2012, 10(6): 441-447.

in vitro comparative study of Bougainvillea spectabilis "stand" leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity

in vitro comparative study of Bougainvillea spectabilis "stand" leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity

  • 摘要: AIM: To study the qualitative analysis of phytochemicals and antibacterial activity of the ethanolic and methanolic extracts of Bougainvillea spectabilis and Bougainvillea variegata leaves. METHODS: Phytochemical constituents were determined qualitatively by the Harborne method, while antimicrobial activities were determined by measuring the zone of inhibition on Mueller Hinton Agar. RESULTS: The maximum inhibitory effects were obtained against the Gram positive microbe Staphylococcus aureus for the methanolic extracts of both B. spectabilis (28.54 0.18) mm and B. variegata (21.97 0.06) mm. The Gram negative microbes Proteus vulgaris (16.00 0.15) mm and Serratia marcescens (16.00 0.06) mm gave maximum inhibitory effects for the ethanolic extracts of B. variegata, while Salmonella typhimurium (17.26 0.12) mm gave a maximum zone of inhibition for the methanolic extract of B. spectabilis.No inhibitory effects were observed for the extracts of B. spectabilis or B. variegate against Enterococcus faecalis, Vibro cholera or Klebsiella pneumoniae. CONCLUSION: Both B. spectabilis and B. variegata possess significant antimicrobial activity that, following additional studies, could replace commercially known antibiotics.

     

    Abstract: AIM: To study the qualitative analysis of phytochemicals and antibacterial activity of the ethanolic and methanolic extracts of Bougainvillea spectabilis and Bougainvillea variegata leaves. METHODS: Phytochemical constituents were determined qualitatively by the Harborne method, while antimicrobial activities were determined by measuring the zone of inhibition on Mueller Hinton Agar. RESULTS: The maximum inhibitory effects were obtained against the Gram positive microbe Staphylococcus aureus for the methanolic extracts of both B. spectabilis (28.54 0.18) mm and B. variegata (21.97 0.06) mm. The Gram negative microbes Proteus vulgaris (16.00 0.15) mm and Serratia marcescens (16.00 0.06) mm gave maximum inhibitory effects for the ethanolic extracts of B. variegata, while Salmonella typhimurium (17.26 0.12) mm gave a maximum zone of inhibition for the methanolic extract of B. spectabilis.No inhibitory effects were observed for the extracts of B. spectabilis or B. variegate against Enterococcus faecalis, Vibro cholera or Klebsiella pneumoniae. CONCLUSION: Both B. spectabilis and B. variegata possess significant antimicrobial activity that, following additional studies, could replace commercially known antibiotics.

     

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