短葶山麦冬皂苷DT-13通过抑制MMP-2/9抑制肺癌A549细胞粘附和侵袭的作用

DT-13, a steroidal saponin from Liriope muscari L. H. Bailey, suppresses A549 cells adhesion and invasion by inhibiting MMP-2/9

摘要: 目的:检测短葶山麦冬皂苷DT-13对肺癌A549细胞粘附及侵袭的作用,及对金属基质蛋白表达的影响。方法:利用MTT法寻找到DT-13合适的作用浓度;利用A549细胞与人脐静脉内皮细胞、纤连蛋白的粘附,评价DT-13对细胞粘附的作用;利用transwell小室,考察DT-13对细胞侵袭的作用;采用Western Blotting方法,检测DT-13对A549细胞中金属基质蛋白-2/9的作用;结论:DT-13在10和30molL-1时,可显著地抑制A549细胞的粘附与侵袭,并且对A549细胞中金属基质蛋白-2和金属基质蛋白-9的表达均有抑制作用。

Abstract: AIM: To present the anti-tumor metastatic activity of the saponin derivative DT-13 on the A549 cell line. METHODS: The effective concentration of DT-13 was determined by the MTT assay; the ability of DT-13 on anti-adhesion was detected with the assistance of HUVECs and fibronectin; the ability of DT-13 on anti-invasion was detected by the transwell plate method; the expression of MMP-2 and MMP-9 was determined by Western blotting. RESULTS and CONCLUSIONS: DT-13 could inhibit the proliferation and adhesion of A549 cells to HUVECs and fibronectin, and the invasion of A549 cells through the extracellular matrix. In addition, DT-13 could inhibit the expression of MMP-2 and MMP-9 in A549 cells.