旱生香茶菜中的三萜成分
Triterpenes from Isodon xerophilus
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摘要: 目的:研究旱生香茶菜的化学成分。方法:采用多种色谱技术和方法进行分离,通过波谱分析鉴定化合物结构。采用MTT法进行人体肿瘤细胞毒体外活性实验。结果:从旱生香茶菜叶中分离并鉴定了10个三萜类化合物:齐墩果酸(1),乌苏酸(2), maslinic acid (3), 2-羟基乌苏酸(4), hederagenin (5), 3, 23-dihydroxy-urs-12-en-28-oic acid (6), arjunolic acid (7), asiaticacid (8), 2, 3, 24-trihydroxyolean-12-en-28-oic acid (9), 2, 3, 24-trihydroxyurs-12-en-28-oic acid (10)。测试了10个化合物对3种(K562, MKN45, HepG2)人体肿瘤细胞株的细胞毒活性。结论:化合物1-10均首次从该植物中分离得到,并讨论了它们的生源关系。所有三萜化合物对上述三种人体肿瘤细胞株都没有细胞毒活性。Abstract: AIM: To investigate the chemical constituents of Isodon xerophilus. METHODS: The constituents were isolated by various chromatographic methods, and the chemical structures were elucidated on the basis of spectral analyses. Their cytotoxicity was evaluated by the MTT method in 96-well microplates. RESULTS: Ten triterpenes were isolated and identified as oleanolic acid (1), ursolic acid (2), maslinic acid (3), 2-hydroxyursolic acid (4), hederagenin (5), 3, 23-dihydroxy-urs-12-en-28-oic acid (6), arjunolic acid (7), asiatic acid (8), 2, 3, 24-trihydroxyolean-12-en-28-oic acid (9), and 2, 3, 24-trihydroxyurs-12-en-28-oic acid (10). All compounds were tested against three human tumor cell lines, K562, MKN45, and HepG2. CONCLUSION: All of the compounds were isolated from this plant for the first time. The proposed biosynthetic pathway of these triterpenes was discussed. All of the triterpenes showed noncytotoxic activity against the above-mentioned three human tumor cell lines.