Abstract: AIM: To investigate the chemical constituents of Allophylus longipes. METHODS: Compounds were isolated and purified by various chromatographic techniques and their structures were elucidated by physicochemical characteristics and spectral data. RESULTS: Twenty-five compounds were isolated and identified as cycloart-24-en-3, 26-diol (1), 3-oxotrirucalla-7, 24-dien-21-oic acid (2), zizyberenalic acid (3), colubrinic acid (4), ent-4(15)-eudesmene-1, 6a-diol (5), 4(15)-eudesmene-1, 8a-diol (6), 4(15)-eudesmene-1, 5a-diol (7), methyl asterrate (8), betulin (9), betulinic aldehyde (10), betulinic acid (11), 3-hydroxy-5a, 8a-epidioxyergosta-6, 22-dien (12), 3-oxo-19a-hydroxyurs-12-en-28-oic acid (13), ursolic acid (14), scopoletin (15), fraxidin (16), cleomiscosin A (17), 4-hydroxy-3-methoxybenzaldehyde (18), 4-hydroxy-3-methoxycinnamaldehyde (19), 2',6'-dihydroxy-4'-methoxyacetophenone (20), p-(aminoalkyl)-benzoic acid (21), 4-hydroxy-3-methoxybenzoic acid (22), 1-O-p-coumaroylglucose (23), -sitosterol (24), and poriferast-5-ene-3, 4-diol (25). CONCLUSION: All the compounds were isolated from Allophylus longipes for the first time.