从腺梗豨莶中分离得到的一种具有细胞毒活性的新氨基甲酸酯

A new carbamate with cytotoxic activity from the aerial parts of Siegesbeckia pubecens

摘要: 目的:对腺梗豨莶(Siegesbeckia pubescens)地上部分进行化学成分的研究。方法:运用萃取、结晶及各种柱层析方法进行分离纯化,并通过理化鉴别和波谱分析鉴定化合物结构。此外,测定了化合物1对A549人肺癌细胞的细胞毒活性。结果:从腺梗豨莶中分离鉴定了4个化合物,分别为(E)-3-(3-oxobut-1-enyl)phenyl dimethylcarbamate(1), ent-2-oxo-15, 16,19-trihydroxypimar-8(14)-ene(2), 16-acetylkirenol(3)和3, 7-dimethylquercetin(4)。结论:化合物1为新化合物, MTT细胞毒活性显示其IC50为58gmL-1。

Abstract: AIM: To study the chemical constituents of Siegesbeckia pubescens. METHODS: The chemical constituents were isolated by extraction, crystallization and various chromatographic methods, and the chemical structures were elucidated on the basis of spectral analysis. In addition, the cytotoxic activity of compound 1 was evaluated using human lung cancer cell A 549. RESULTS: Four compounds were obtained, and their structures were identified as (E)-3-(3-oxobut-1-enyl)phenyl dimethylcarbamate (1), ent-2-oxo-15, 16, 19-trihydroxypimar-8(14)-ene (2), 16-acetylkirenol (3), 3, 7-dimethylquercetin (4). CONCLUSION: Compound 1 is a new carbamate, and the IC50 in MTT method of compound 1 was 58 gmL-1.