苏木种子中两个新的卡山烷型二萜

Two new cassane diterpenoids from the seeds of Caesalpinia sappan Linn.

摘要: 目的:对苏木的种子进行化学成分研究。方法:采用各种柱层析方法,从苏木种子分离得到化合物,并通过波谱方法确定其化学结构。此外,运用MTT分析法测定了这些化合物对SF-268, MCF-7和HepG2细胞株的细胞毒活性。结果:从苏木种子中分离鉴定了4个卡山烷型二萜,分别为phanginin L (1), phanginin M (2), phanginin I (3), phanginin G (4)。结论:Phanginin L (1) and phanginin M (2)为新化合物。化合物1对HepG2细胞株有中等抑制活性,其IC50为9.13gmL-1。

Abstract: AIM: To study the chemical components of the seeds of Caesalpinia sappan. METHODS: Compounds were isolated and purified by column chromatography. Their structures were elucidated on the basis of spectroscopic methods. In addition, the cytotoxic activity of these compounds were evaluated using SF-268, MCF-7 and HepG2 cell lines by the MTT assay. RESULTS: Four cassane diterpenoids were obtained and their structures were identified as phanginin L (1), phanginin M (2), phanginin I (3), phanginin G (4). CONCLUSION: Phanginin L (1) and phanginin M (2) are new cassane diterpenoids. Compound 1 showed moderate cytotoxicity against HepG2 cell line with IC50 of 9.13 gmL-1.