丹参酮IIA在3T3-L1脂肪细胞中促进脂联素的组装
Tanshinone IIA promotes the assembly of adiponectin in 3T3-L1 adipocytes
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摘要: 目的:研究丹参酮IIA对3T3-L1脂肪细胞中脂联素表达和蛋白多聚化的作用。方法:用油红染色鉴定3T3-L1脂肪细胞的分化状态。用Real-time PCR检测基因的mRNA表达水平。用2%-15%梯度SDS-PAGE及Western blot检测脂联素三种聚体。用siRNA技术进行基因沉默。结果:本研究发现传统中药丹参中的萘醌二萜类成分丹参酮IIA在脂肪细胞中激活AMPK通路,同时抑制脂肪细胞分化,降低脂联素的表达。而在前体脂肪细胞分化过程或成熟脂肪细胞中加入丹参酮IIA进行处理,均明显促进脂联素的多聚化,增加高聚体的浓度。抑制AMPK通路能够解除丹参酮IIA对脂联素的作用。结论:丹参酮IIA通过激活AMPK在3T3-L1脂肪细胞中促进脂联素的组装。Abstract: AIM: To investigate the effect of tanshinone IIA on adiponectin biosynthesis. METHODS: The differentiation state of 3T3-L1 adipocytes was checked by Oil Red O staining. mRNA levels were examined by real-time PCR. Adiponectin oligomers were subjected to 2%15% gradient SDS-PAGE and then detected using western blotting. Gene silencing experiments were performed using standard siRNA methods. RESULTS: It was found that tanshinone IIA, a diterpenoid naphthoquinone from the Chinese traditional herb Danshen (Salvia miltiorrhiza), inhibited the differentiation of preadipocytes and down-regulated the expression of adiponectin. In contrast, tanshinone IIA promoted the assembly of high-molecular weight (HMW) adiponectin during differentiation and in mature adipocytes. Tanshinone IIA activated AMP-activated protein kinase (AMPK) in 3T3-L1 adipocytes, and suppression of the AMPK pathway blocked the effect of tanshinone IIA on adiponectin expression and multimerization. CONCLUSION: These data suggest that tanshinone IIA promotes the assembly of HMW adiponectin through a process involving AMPK activation.