Abstract: AIM: To study the constituents in the roots of Achyranthese bidentata Bl. METHODS: D-101 porous polymer resin column, normal-phase silica gel column chromatography, revised-phase medium pressure column chromatography, Sephadex LH-20 and prep-HPLC were used for the separation, and spectroscopic techniques were employed for the identification of the isolated compounds. RESULTS: Five saponins were isolated and elucidated as achyranthoside C (1), achyranthoside D (2), chikusetsusaponin Ⅳ (3), ginsenoside Ro (4) and zingibroside R1 (5). CONCLUSION: Compound 1 was obtained from natural source for the first time, and compound 3 was reported from the family of Amaranthaceae for the first time.