Abstract:
Steroid saponins are secondary metabolites with multiple medicinal values that are found in large quantities in natural medicines, especially
Vernonia amygdalina, a famous nature medicine for the treatment of tonsillitis, diabetes, pneumonia. The current study was designed to combine molecular networking (MN) with diagnostic ions for rapid identification of Δ
7,9(11) stigmastane-type saponins which were the
α-glucosidase inhibitory active substances in
V. amygdalina. First, the
α-glucosidase inhibitory activities of five Δ
7,9(11) stigmastane-type steroid saponins that were previously isolated were screened, which indicated that the Δ
7,9(11) stigmastane-type steroid saponin was one of the active constituents responsible for ameliorating diabetes. Furthermore, a strategy was proposed to identify stigmastane-type steroid saponins and verify the plausibility of derived fragmentation pathways by applying MN, MolNetEnhancer and unsupervised substructure annotation (MS2LDA). Based on this strategy, other seven Δ
7,9(11) stigmastane-type steroid saponins were identified from this plant. Our research provide scientific evidence for the antidiabetic potential of the steroid saponin-rich extract of
V. amygdalina leaf.