Abstract:
Genome mining for the search and discovery of two new globin-like enzymes, TriB from
Fusarium poae and TutaA from
Schizophyllum commne, are involved in the synthesis of two linear terpenes tricinonoic acid (
1 ) and 2-butenedioic acid (
3 ). Both
in vivo heterologous biosynthesis and
in vitro biochemical assays showed that these two enzymes catalyzed the C-C double bond cleavage of a cyclic sesquiterpene precursor (−)-germacrene D (
7 ) and a linear diterpene backbone schizostain (
2 ), respectively. Our work presents an unusual formation mechanism of linear terpenes from fungi and expands the functional skills of globin-like enzymes in the synthesis of terpene compounds.