Abstract:
Thibetanosides E-H (1-4), four new steroidal constituents including three rare sulfonates (
2-4 ), were isolated from the roots and rhizomes of
Helleborus thibetanus, together with nine known steroidal compounds (
5-13 ). Their structures were elucidated by detailed spectroscopic analysis, including 1D and 2D NMR techniques and chemical evidence. In this study, compounds
2-13 were evaluated for their cytotoxic activities against HCT116, A549 and HepG2 tumor cell lines
in vitro. Among them, compound
8 (thibetanoside C) showed cytotoxicities against A549 cells (IC
50 39.6±1.9 μmol·L
-1) and HepG2 cells (IC
50 41.5±1.1 μmol·L
-1), respectively. Compound
9 (23S, 24S)-24-(
O-
β-D-fucopyranosyl)oxy-3
β, 23-dihydroxy-spirosta-5, 25(27)-diene-1
β-yl
O-(4-
O-acetyl-
α-L-rhamnopyranosyl)-(1→2)-
O-
β-D-xylopyranosyl-(1→3)-
α-L-arabinopyranoside) showed cytotoxicity against HCT116 cells (IC
50 33.6±2.1 μmol·L
-1).
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