Abstract: Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22(N), 23, 25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside ( 1 ) and 26-O-β-D-glucopyranosyl-(25R)-5α-furost-20(22)-en-3β, 26-diol ( 2 ), together with 7 known ones including 26-O-β-D-glucopyranosyl-(25R)-5, 20(22)-dien-furost-3β, 26-diol ( 3 ), (25R)-5-en-spirost-3β-ol-O-β-D-glucopyranosyl-(1→4)-α-L-rhmanopyranosyl-(1→2)-β-D-galactopyranoside ( 4 ), funkioside D ( 5 ), aspidistrin ( 6 ), tigogenin-3-O-β-D-lucotrioside ( 7 ), desglucolanatigonin Ⅱ ( 8 ), and degalactotigonin ( 9 ), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides ( 6 ) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.