Abstract:
Four new triterpene saponins, mandshunosides F-I (
1-4 ), together with five known compounds (
5-9 ), were isolated from the roots and rhizomes of
Clematis mandshurica. Their structures were elucidated on the basis of spectroscopic evidences and hydrolysis products. Bisdesmosidic saponin (
3-9 ) showed modest suppression of NO production with the inhibition ratios in the range of 51.3%-64.6% at 50 μmol·L
-1, whereas monodesmosidic saponins with a free carboxyl group at C-28 (
1 and
2 ) showed potent inhibitory activities with IC
50 values being 12.7 and 8.3 μmol·L
-1, respectively.