Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application

AIM:A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2.METHODS:Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2.The monoclonal antibody was purified by protein G affinity,whereas the polyclonal antibody was purified by protein A affinity.The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples.RESULTS:A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2,highly specific monoclonal and polyclonal antibodies.This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples.CONCLUSIONS:The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit.