Gastroprotective effect of the iridoid fraction from Barleria prionitis leaves on experimentally-induced gastric ulceration

AIM:To study the gastroprotective effect and in vivo antioxidant potential of a standardized iridoid fraction from B.prionitis leaves(BPE) against different gastric ulcer models in rats.METHOD:The standardized iridoid fraction from BPE at 50,100,and 200 mg/kg body weight was administered orally,twice daily for 5 days for prevention from aspirin,ethanol,cold-restraint stress(CRS),and pylorus ligation(PL)-induced ulcers.Estimation of the antioxidant enzyme activity was carried out in a CRS-induced ulcer model,and various gastric secretion parameters including volume of gastric juice,acid output,and pH value were estimated in the PL-induced ulcer model.RESULTS:BPE showed a dose-dependent ulcer protective effect in PL(18.67%-66.26% protection),aspirin(24.65%-63.25% protection),CRS(20.77%-59.42% protection),and EtOH(16.93%-77.04% protection)-induced ulcers.BPE treatment in PL-rats showed a decrease in acid-pepsin secretion,and enhanced mucin and mucosal glycoproteins.However,BPE reduced the ulcer index with significant decrease in LPO(P0.01-0.001),SOD(P0.01-0.001),and an increase in CAT(P0.01-0.001),activity in the CRS-induced model.CONCLUSION:The data shows that the iridoid fraction from BPE possesses anti-ulcerogenic and antioxidant potential.