A new saikogenin from the roots of Bupleurum bicaule

AIM:To study the chemical constituents from the roots of Bupleurum bicaule Helm(Apiaceae).METHOD:Silica gel,Sephadex LH-20,MPLC Rp-C18 column chromatography,and HPLC were used for isolation of compounds.The structures were elucidated on the basis of 1D-and 2D-NMR technology and HRESI-MS.Compounds were evaluated in vitro for their inhibitory ability against the proliferation of rat mesangial cells by the MTT method.RESULTS:Twelve compounds were isolated,and their structures were identified on the basis of their spectroscopic and ico-chemical properties as 13,28-epoxy-olean-11-en-3-one(1),saikogenin E(2),saikogenin G(3),11-methoxy-3,16,23,28-tetrahydroxyolean-12-ene(4),saikogenin D(5),prosaikogenin F(6),prosaikogenin A(7),prosaikogenin G(8),prosaikogenin D(9),laccaic acid(10),methyl gallate(11),and ethyl gallate(12).Compounds 1,2,7,8,and 10 were observed to have inhibitory activity against mesangial cell proliferationin to different degrees.CONCLUSION:Compound 1,8,and 10 exhibit significant inhibitory effects on rat mesangial cell proliferation induced by Ang II.