F-01A,an antibiotic,inhibits lung cancer cells proliferation

AIM:In an effort to identify novel,small molecules which can affect the proliferation of lung cancer cells,F-01A,a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested.METHOD:F-01A was tested for its antitumor properties on the lung cancer cell line SPC-A-1,at six doses(0.1,0.5,1,2.5,and 5 molL-1),using various cellular assays.Cell viability was measured by the MTT assay,Hochest 33258 was used to study nuclear morphology;DNA ladder and the loss of mitochondrial membrane potential were also evaluated.RESULTS:F-01A induces apoptosis against SPC-A-1 cells in a dose-dependent manner.The IC50 is 0.65 molL-1,and the inhibition at 5 molL-1 is 87.89%.Further,JC-1 staining indicates F-01A could induce the loss of mitochondrial membrane potential,and the DNA fragment is evident.CONCLUSION:Mechanistic analysis showed that F-01A induced apoptosis of cancer cells probably in the mitochondrial pathway.The antitumor actions of F-01A involve activation of the apoptotic pathway against SPC-A-1 cells,and it may be valuable for further drug development.