Angio-suppressive triterpenoids from Ardisia cf.elliptica(subgenus Tinus) on duck(Anas platyrynchos L.) chorioallantoic membrane

The dichloromethane extract of the air-dried leaves of Ardisia cf.elliptica(subgenus Tinus) afforded a mixture of bauerenol(1a),-amyrin(1b) and -amyrin(1c).Their structures were identified by NMR spectroscopy.Mixtures of the triterpenes(1a-1c) at ratios of 2:2:1,2:2:3 and 1:1:1 were tested for their angio-suppressive effects on duck chorioallantoic membrane(CAM).All three ratios were found to be effective in restricting inter-capillary length,while 1a-1c(2:2:1) was most effective in reducing branch point density with 100% CAM viability and embryo survivability,suggesting a high impact angio-suppressive potential of 1a-1c(2:2:1).