Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies

AIM:To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.METHODS:Eight rats were given 5 mg·kg-1 isorhamnetin-3-O-neohesperidoside after intravenous administration.A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard.The analytes and rutin(internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L-1 ammonium acetate buffer/methanol(20:80,V/V) on a C18 column(150 mm×2.1 mm,I.D.,5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.RESULTS:The assays were linear over the concentration range of 0.01-10 μg·mL-1 for isorhamnetin-3-O-neohesperidosidein rat plasma.The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL-1.CONCLUSION:The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.