The Spectrum-Effect integrated fingerprint of Polygonum cuspidatum based on HPLC-diode array detection-flow injection-chemiluminescence

AIM:To establish the Spectrum-Effect integrated fingerprint of Polygonum cuspidatum to evaluate the quality of P.cuspidatum.METHODS:An on-line HPLC-DAD-flow injection chemiluminescence(FICL) method was developed to investigate the quality of P.cuspidatum from different habitats based on the established Spectrum-Effect integrated fingerprint.RESULTS:Nineteen batches of samples of P.cuspidatum were evaluated for the similarity of their chromatographic and free radical scavenging fingerprints,and the results compared.Main antioxidants were estimated by regression analysis between peak areas of thirteen compounds and their activities.Some active compounds were identified by HPLC-ESI-MS.CONSULSIONS:The results indicated that main antioxidants in P.cuspidatum could be rapidly screened by the established Spectrum-Effect integrated fingerprint based on on-line HPLC-DAD-FICL,and would be more efficient and objective method to evaluate the quality of P.cuspidatum.