Screening and analysis of metabolic components in rat urine and feces after oral administration of total glycosides of Jinkuishenqi pills by HPLC-ESI/MSⁿ

AIM:To identify the chemical components of the total glycosides(TG) of Jinkuishenqi pills and their metabolites in vivo.METHODS:An HPLC-ESI-MS method was developed for analysis of the metabolites of TG in rat plasma,after the rat was administered with the TG.RESULTS:A total of 18 compounds were detected in urine after oral administration of TG,nine of which are metabolites and the others are the original form of the compounds contained in Jinkuishenqi pills.Among them,the metabolites are gallic acid,dehydromorroniaglycone,2-hydroxyacetophenone-4-O-glucuronic acid esters,paeonimetabolin,Mlog Ⅰ-2,Mlog-1,2-hydroxy-4-methoxy acetophenone-5-O-sulfate,2,4-dihydroxyacetophenone and one unknown compound,respectively.A total of 20 compounds were detected in the feces after oral administration of TG,eight of which are metabolites and the others are the original form of the compounds contained in Jinkuishenqi pill.Among them,the metabolites are gallic acid,swerosimetabolin A,paeonimetabolin,Mlog Ⅰ-2,Mmor-2,Mlog-1 and two unknown compounds.CONCLUSIONS:Using HPLC-ESI-MS,it was possible to screen and analyze the multiple compounds and metabolites of TG in vivo of rats.The identification and structure elucidation of these metabolites provided essential data for further pharmacological and mechanism of effectiveness of TG.