Chemical constituents from the aerial parts of Angelica sinensis and their bioactivities

AIM: To investigate the chemical constituents from the aerial parts of Angelica sinensis and their anticoagulative and antibiotic activities. METHODS: The constituents of the 80% ethanol extract were isolated and purified by various chromatographic methods. Their structures were identified by the physicochemical characteristic and spectral data. The anticoagulative activity of the isolated compounds were tested using the thrombin time method assay, and the antibiotic activity were tested using the serial two-fold dilution method. RESULTS: Thirteen known compounds were isolated and identified as 24, 24-dimethyl-9,19-cyclolanostan-3-ol (1), -sitosterol (2), 2, 3, 6-trimethylbenzoic acid (3), uracil (4), daucosterol (5), hyperoside (6), allantoin (7), D-mannitol (8), 1S-(4'-hydroxyphenyl)-1, 2-ethanediol (9), caffeic acid (10), chlorogenic acid (11), sucrose (12) and D-glucose (13). Compounds 3, 4, 6, 7, 9, 10 and 11 moderately prolonged thrombin time. Compounds 1, 4, 6, 7 and 9 significantly inhibited the growth of Aeromonas hydrophila. CONCLUSION: Compounds 1, 3, 6-9 were isolated from this plant for the first time. Compounds 1, 3, 4, 6, 7, 9, 10 and 11 are the important active constituents of the aerial parts of Angelica sinensis.