Combining label-free quantitative proteomics and 2D-DIGE to identify the potential targets of Sini decoction acting on myocardial infarction

Sini decoction (SNT) is a traditional formula for its efficacy in warming the spleen and stomach and dispersing cold. However, due to the complexity of its multiple components, unraveling the mechanism of action of SNT persists as a challenge. In this study, we employed a synergistic approach combining two-dimensional fluorescence difference gel electrophoresis (2D-DIGE)-based drug affinity responsive target stability (DARTS) with label-free quantitative proteomics techniques to pinpoint the direct and indirect protein targets of SNT acting on myocardial infarction. A comprehensive analysis identified a total of 590 proteins. Comparing the SNT group with the model group, we observed significant upregulation of 30 proteins and downregulation of 51 proteins. Notably, through the integration of 2D-DIGE DARTS with proteomics data and pharmacological assessments, our findings suggest that protein disulfide-isomerase A3 (PDIA3) may emerge as a potential protein target through which SNT exerts its protective effects on myocardial cells in the context of myocardial infarction.