New triterpenoid saponins from the leaves of Ilex chinensis and their hepatoprotective activity

Seven new triterpenoid saponins, including five ursane-type saponins, ilexchinenosides R–V ( 1 – 5 ), and two oleanane-type saponins, ilexchinenosides W–X ( 6 – 7 ), with four known triterpenoid saponins ( 8 – 11 ) were isolated from the leaves of Ilex chinensis. Their structures were elucidated by comprehensive spectroscopic 1D and 2D NMR and HR-ESI-MS data. Their sugar moieties were determined by HPLC analysis compared with standards after hydrolysis and derivatization. Compounds 1 , 2 , 4 , 9 and 10 exhibited potential hepatoprotective activity against N-acetyl-p-aminophenol (APAP)-induced HepG2 cell injury in vitro.