LUO Yu, ZENG Lin-Jie, LIU Xiao-Qin, LI Lu, ZENG Qi-Yan. cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus [J]. Chin J Nat Med, 2021, 19(2): 81-89. DOI: 10.1016/S1875-5364(21)60009-2
Citation: LUO Yu, ZENG Lin-Jie, LIU Xiao-Qin, LI Lu, ZENG Qi-Yan. cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus [J]. Chin J Nat Med, 2021, 19(2): 81-89. DOI: 10.1016/S1875-5364(21)60009-2
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cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus

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    • Abstract
  • We isolated a novel lectin (AHL) from Artocarpus hypargyreus Hance and showed its immunomodulatory activities. In this study, the amino acid sequence of AHL was determined by cDNA sequencing. AHL cDNA (875bp) contains a 456-bp open reading frame (ORF), which encodes a protein with 151 amino acids. AHL is a new member of jacalin-related lectin family (JRLs), which share high sequence similarities to KM+ and Morniga M, and contain the conserved carbohydrate binding domains. The antitumor activity of AHL was also explored using Jurkat T cell lines. AHL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bad and Bax up-regulation, and caspase-3 activation. We further showed that the activation of ERK and p38 signaling pathways is involved for the pro-apoptotic effect of AHL.
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